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. 2013 Sep 24;123(10):4195–4207. doi: 10.1172/JCI62891

Figure 7. SALL4 interacts with MLL.

Figure 7

(A) Co-IP with anti-SALL4 antibody was done using 293T cell lysates after SALL4 overexpression. The protein complex from Co-IP was resolved in SDS-PAGE and immunoblotted using antibodies against SALL4, RbBP5, and Menin. (B) MLL full-length and truncated proteins (MLL-BP and MLL-N). (C) 293T cells were cotransfected with SALL4 and Flag-tagged truncated MLL constructs. IP was performed with Flag antibody, followed by Western blot analysis with SALL4 antibody, which demonstrated that SALL4 could only be detected in pulldown with MLL-BP. Flag antibody could pull down both overexpressed MLL-BP and MLL-N proteins from 293T transfected cells. (D) SALL4 truncated vectors used for interaction with MLL-BP. (E) 293T cells were cotransfected with various SALL4 vectors and Flag-tagged truncated MLL-BP constructs. IP was performed with MLL antibody, followed by Western blot analysis with SALL4 antibody. Whereas the C terminus of SALL4 (C-1581) did not interact with MLL-BP, the N terminus (N-523) retained its ability to bind to MLL-BP.