Skip to main content
. 2013 Sep 3;123(10):4390–4404. doi: 10.1172/JCI65856

Figure 8. miR-9 promotes nuclear translocation of β-catenin that contributes to ABCB1 overexpression.

Figure 8

(A) Immunofluorescence of β-catenin (green) showing membrane staining in a primary K15.KrasG12D.Smad4–/– SCC. Membrane staining was lost in the passaged SCC and had increased nuclear β-catenin. SCC derived from B911-GFP cells shows cytoplasmic β-catenin, which became predominately nuclear in a B911-miR-9 tumor. A B931-GPF tumor shows cytoplasmic and sporadic nuclear β-catenin, whereas a B931-Zip-9 tumor shows patchy restoration of membrane-associated β-catenin. Sections were counterstained with DAPI (blue). Scale bar: 50 μm. (B) qRT showing siRNA knockdown of β-catenin in B911-GFP and B911-miR-9 cells. *P = 0.03 compared with scrambled siRNA control (n = 3). (C) Knockdown of β-catenin reduces ABCB1a expression. *P = 0.01 compared with B911-GFP transfected with scrambled siRNA (n = 3). #P = 0.03 compared with B911-miR-9 transfected with β-catenin siRNA (n = 3).