Figure 6. Overexpression of α-actinin-2 in mouse muscle increases RCAN1-4 expression.

(A and B) Overexpression of α-actinin-2 (3.1-fold) through i.m. injection of 1 × 10¹¹ vector genomes (vg) of rAAV6-CMV-mActn2 resulted in a 2.7-fold increase in RCAN1-4 expression (P = 0.028). (C and D) In contrast, a 1.5-fold overexpression of α-actinin-3 through injection of 1 × 10¹¹ vg of rAAV6-CMV-mActn3 did not significantly alter RCAN1-4 expression in injected muscles (mean ± SEM; *P < 0.05, Mann-Whitney U test; n = 4 for all samples). In A and B, the lanes were run on the same gel but were noncontiguous.