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. 2013 Sep 3;123(10):4158–4169. doi: 10.1172/JCI69003

Figure 2. FKBP51 and Hsp90 synergistically prevent tau degradation by the 20S proteasome.

Figure 2

(A) Tau was incubated alone, with 20S proteasome, or with FKBP51 and 20S proteasome for 5 minutes. (B) Tau was incubated alone, with 20S proteasome, with FKBP51, and with Hsp90 with or without FKBP51 for 5 minutes. (C) Relative tau levels (± SEM) in each sample using 1 μM Hsp90. **P < 0.01, ***P < 0.001. (D) Tau levels (± SEM) following incubation with FKBP51 and Hsp90 in the presence of the 20S proteasome (green). Calculated tau levels (blue) from the addition of the results of tau preserved following incubation with Hsp90 and with the tau preserved following incubation with FKBP51 in the presence of the 20S proteasome. *P < 0.05. (E) FKBP51 was incubated with 100 μg Suc-LLVY-AMC and 20S proteasome for 5 minutes. OD readings (± SEM) were taken at 460 nm.