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. 2013 Sep 3;123(10):4158–4169. doi: 10.1172/JCI69003

Figure 6. FKBP51 expression increases with age, altering Hsp90 complexes.

Figure 6

(A) Average mRNA expression (± SEM) from the SFG, PCG, EC, and HPC of young (20–59 years old) and aged (69–99 years old) samples. ***P < 0.0001. (B) The average FKBP5 mRNA expression from all 4 regions for each sample was plotted against age. P < 0.0001; r = 0.4916. (C) Human brain homogenates from the PCG of 4 males (ages 21, 42, 85, and 95 years) were analyzed by Western blot by FKBP51, Hsp90, FKBP52, CHIP, and GAPDH antibodies. (D) Quantification of protein levels with aging from multiple blots. FKBP51, P = 0.0765, r = 0.8528; Hsp90, P = 0.394, r = 0.3672; FKBP51, P = 0.9632, r = 0.001352; CHIP, P = 0.0269, r = 0.9469. (E) Hek cells transfected with Tau, FKBP51, Hsp90, and CHIP, respectively, were harvested after 48 hours. Following mouse anti-Hsp90 antibody immunoprecipitation, samples were analyzed by Western blot for FKBP51, CHIP, Hsp90, Tau 12 (total tau), and GAPDH antibodies, respectively.