Figure 4. IL-17A– and IFN-γ–producing DETC subsets maintain their CD27^–45RB⁺ phenotype.

(A) Phenotypic characterization of freshly isolated WT and Il17a^–/– DETC. Cells are gated on Vγ3⁺Thy1.2⁺ and CD45RB, CD27, NK1.1, CD62L, CD122, IL-7R, and CD69 expression (solid lines), and their appropriate IgG controls (gray) were measured by flow cytometry. (B) Distinct DETC subsets produce IL-17A and IFN-γ. Intracellular staining on DETC short-term cell lines stimulated with anti-CD3ε (10 μg/ml) for IL-17A and IFN-γ production. Cells are gated on Vγ3⁺Thy1.2⁺. (C) IL-17A– and IFN-γ–producing DETC are CD27^–CD45RB⁺. Fresh epidermal cell suspensions were stimulated with anti-CD3ε (10 μg/ml), and expression of CD27, CD45RB, and intracellular IL-17A and IFN-γ were measured by flow cytometry. Cells are gated on IL-17A⁺Vγ3⁺Thy1.2⁺ and IFN-γ⁺Vγ3⁺Thy1.2⁺, respectively.