(A) Representative kidney sections of CX3CR1GFP/+ reporter mice and CX3CR1GFP/GFP-deficient mice (original magnification, ×200). Arrows point to GFP+ cells. (B) CD11c versus MHC-II staining of CD45+GFP+ cells from CX3CR1GFP/+ reporter mice (left) and quantification of DCs (MHC-II+CD11c+) and MPs (MHC-II+CD11c–) (right) in CX3CR1GFP/+ (GFP/+) and CX3CR1GFP/GFP mice (GFP/GFP). Results of 5 individual experiments were combined. (C) Absolute numbers of GFP+ DCs in various organs and tissues of CX3CR1GFP/+ and CX3CR1GFP/GFP mice, given as total DCs per organ. Results are representative of 2 individual experiments, with 3 to 4 mice per group. ingLN, inguinal LN. (D) Relative CX3CL1 expression in different organs compared with expression levels in the kidneys. Statistical significance was tested with (B) unpaired Student’s t test or (C and D) 1-way ANOVA with Bonferroni multiple-comparison test. **P < 0.01, ***P < 0.001.