Figure 4. CX₃CR1 deficiency attenuates NTN.

(A) Kidney sections of CX₃CR1^+/+ and CX₃CR1^GFP/GFP animals 15 days after NTS challenge (Masson trichrome; original magnification, ×200). (B) Percentage of crescentic glomeruli in histological sections of CX₃CR1^+/+ and CX₃CR1^GFP/GFP mice on day 15 after disease induction. (C) Tubular casts per high-power field (hpf) (original magnification, ×100) in CX₃CR1^+/+ and CX₃CR1^GFP/GFP mice 15 days after NTS challenge. (D) Representative microphotographs of immunostained F4/80⁺ cells in kidney cortices from CX₃CR1^+/+ and CX₃CR1^GFP/GFP mice on day 15 after NTN induction (original magnification, ×200). (E) Quantification of infiltrating F4/80⁺ cells in CX₃CR1^+/+ and CX₃CR1^GFP/GFP animals 15 days after NTS challenge. (F) Immunofluorescence microscopy of kidney sections from nephritic CX₃CR1^GFP/+ and CX₃CR1^GFP/GFP mice on day 10 after NTN induction (red [autofluorescence], green [GFP]). Scale bar: 50 μm. Data points represent individual mice, and (D–F) results are representative of 2 individual experiments, with 12 to 16 mice per group. Statistical significance was tested by 2-tailed Mann-Whitney test.