Table 1. Relative rate parameters of plasmid transfer.
Plasmid in donor strain(a) | Donor cfu/ml (D) | Recipient S. epidermidis strain(b) | Recipient cfu/ml (R) | Trans-conjugants cfu/ml (T) | Frequency of conjugation (T/R) | Γ = T/(D×R) |
pG0400 | 4.8×109 | Δcrispr | 1.3×109 | 8.0×104 | 6.2×10−5 | 1.3×10−14 |
4.1×109 | 1.1×109 | 1.9×104 | 1.7×10−5 | 4.2×10−15 | ||
pG0400 | 1.3×1010 | RP62a | 1.5×109 | 1.4×101 | 9.3×10−9 | 7.2×10−19 |
1.6×1010 | 1.0×109 | 2.3×101 | 2.3×10−8 | 1.4×10−18 | ||
pG0400mut | 8.0×109 | Δcrispr | 1.3×109 | 1.5×105 | 1.2×10−4 | 1.4×10−14 |
1.1×1010 | 1.7×109 | 5.0×104 | 2.9×10−5 | 2.7×10−15 | ||
pG0400mut | 1.6×1010 | RP62a | 2.0×109 | 2.4×104 | 1.2×10−5 | 7.5×10−16 |
8.0×109 | 1.4×109 | 4.0×104 | 2.9×10−5 | 3.6×10−15 |
pG0400mut is a derivative of pG0400 containing 9 silent mutations in the spc1 CRISPR target.
RP62a is a wild-type strain. Δcrispr is an isogenic mutant lacking the CRISPR array.