Figure 7.

JAZ1 and JAZ4 Repress ICE1 Transcriptional Function.

(A) Transient expression assays showed that JAZ1 and JAZ4 repress transcriptional activation of ICE1. GFP fluorescence was detected 48 h after coinfiltration with the indicated constructs after cold treatment (4°C for 4 h). The experiment was repeated three times with similar results.

(B) Quantitative analysis of GFP fluorescence intensity in the transformed and cold-treated plants. Fifty independent fluorescent spots were assessed for fluorescence intensity. Error bars represent se.

(C) qRT-PCR analysis of the accumulation of CBF3-GFP transcripts. Total RNAs were extracted from leaves of N. benthamiana coinfiltrated with combinations of various constructs in (A). The N. benthamiana ACTIN gene was used as an internal control. Error bars show sd from three independent RNA extractions.