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. 2013 Aug 12;25(8):2944–2957. doi: 10.1105/tpc.113.114009

Figure 4.

Figure 4.

WRKY6 Responds to As(V) and Represses the Pi Transporter PHT1;1.

(A) Kinetic study of PHT1;1 and WRKY6 expression by qRT-PCR in wild-type plants exposed to 30 μM As(V). Values show mean ± sd.

(B) qRT-PCR expression analysis of WRKY6 in wild-type plants in response to 30 μM As(V) pulses [±As(V)] or in response to 30 μM Pi pulses (±Pi); duration of each pulse and gap was 1.5 h. Values show mean ± sd.

(C) qRT-PCR of PHT1;1 transcript in wild-type plants (Col-0), in the WRKY6-GFP–overexpressing line (OXWRKY6), and in wrky6-TDNA line grown in +Pi medium for 7 d, transferred to -Pi for 2 d and then to -Pi medium alone or with 20 μM As(V) (1.5 h). In the case of WRKY6-overexpressing lines, values show data from analysis of 10 independent lines. Values show mean ± sd.

(D) ChIP assay of WRKY6-GFP seedlings and PHT1;1 promoter PCR amplification analysis. qPCR of ARE-containing fragments of the PHT1;1 promoter. Enrichment was calculated relative to wild-type plants. ACT8 was used as negative control. Values show mean ± sd. *P < 0.05 (Student’s t test).