Figure 8.

Activation of As(V)-Responsive Transposable Elements Is Independent of Changes in H3K9me2 and H3Ac Levels.

ChIP analysis of As(V)-induced transposable elements in Arabidopsis Col-0 plants grown on 1 mM Pi medium for 7 d, transferred to -Pi medium for 2 d and finally to liquid -Pi medium, alone or supplemented with 30 μM As(V). The assay was performed using antibodies specific for H3K9me2 (A) or H3Ac (B), histone marks associated with repressive and active transcription, respectively. Levels of histone modifications in the genomic regions of As(V)-responsive transposable elements are represented relative to Ta3 in the case of H3K9me2 or to TUB8 for H3Ac. Values show mean ± sd.