Figure 6.

ap2m-1 Mutant Plants Have Low Levels of Auxin in Anthers and Filaments.

(A) Defects in elongation of stamen filaments in ap2m-1 and eir1-4 plants. Images of a whole flower were taken from wild-type (WT), ap2m-1, and eir1-4 plants grown on soil for 6 weeks. Bar = 1 cm.

(B) Quantification of defective flowers in wild-type, ap2m-1, and eir1-4 plants. Flowers that had abnormal growth of filaments were counted. Error bar indicates se (n = 300 flowers). Statistical analysis was performed between the wild type and ap2m-1 and eir1-4 mutants using Student’s t test (*P < 0.01).

(C) Expression of DR5rev:GFP in stamens in wild-type, ap2m-1, and eir1-4 plants. The DR5rev:GFP transgenic plants were crossed with ap2m-1 or eir1-4, respectively, and the expression levels of GFP were examined in wild-type background or the indicated mutant backgrounds. The stamens of wild-type, ap2m-1, and eir1-4 plants were also treated with the indicated chemicals for the indicated periods of time, and DR5rev:GFP expression was examined. Bars = 50 μm (top panels) and 100 μm (bottom panels).

(D) Quantification of GFP signals intensity in wild-type, ap2m-1, and eir1-4 plants. Fluorescence signal intensity of the whole stamens shown in (C) (top panels) was measured using LSM 510 (Zeiss) and expressed as relative values to that of the wild-type plants. Error bar indicates se (n = 50 stamens). Statistical analysis was performed between the wild type and ap2m-1 and eir1-4 mutants using Student’s t test (*P < 0.01).