Confocal Imaging and FRAP Analysis of the MyoB1-YFP–Tagged Material in the Leaf Epidermal Cells of the Columbia or Myosin XI Gene Knockout Plants.
(A) The MyoB1-YFP localization patterns in the genetic backgrounds of control Columbia, xi-k, and xi-k xi-1 xi-2 plants.
(B)
FRAP analysis of the Columbia and xi-k xi-1 xi-2 plants. UB, unbleached; B-Col, bleached Columbia; B-3KO, bleached xi-k xi-1 xi-2 triple knockout.
(C) Consecutive confocal images of the Columbia MyoB1-YFP epidermal cells before (left panel) and after photobleaching. The photobleached area (dashed line) and times after treatment are shown.