Figure 1.

IRT1 Accumulation in the idf1-1 Mutant.

(A) Fourteen-day-old wild-type (Wt) and idf1-1 plants were grown on half-strength MS or Fe-deficient conditions for 3 d (-Fe) and then resupplied with Fe for 6, 12, 24, and 48 h. IRT1 was detected by the anti-IRT1 antibody (IRT1). CB, Coomassie blue stain.

(B) Real-time quantitative RT-PCR monitoring of IRT1 expression in roots of the wild type and idf1-1 under half-strength MS and deficient conditions (-Fe). The expression of ubiquitin21 (UBC21) was used as an internal control. Means and error bars (se) are calculated from four biological repeats.