Figure 9.

Proteins in Cytosolic, Microsome, and Plasma Membrane–Enriched Fractions.

Soluble, microsome, and plasma membrane proteins in the root were isolated from 14-d-old wild type (Wt) and idf1-1 treated for 3 d in Fe-sufficient (C) or -deficient conditions (-Fe). IRT1 was detected with the IRT1 antibody. Anti-cFBPase, anti-SAR1, and anti-H⁺-ATPase antibodies (Agrisera antibodies AS04-043, AS08-326, and AS07-260) were used for the detection of the cytosolic protein cFBPase, endosome protein SAR1, and the plasma membrane protein H⁺-ATPase, respectively. The corresponding proteins are indicated with arrowheads.