Table 1. Half-Life of IRT1 Protein in Fe Resupply.
IRT1 t1/2 (h) |
||
---|---|---|
Treatments | Wild Type | idf1-1 |
CHX | 15.0 ± 0.6 | 17.0 ± 0.7 |
CHX/MG132 | 20.0 ± 0.2 | 21.6 ± 0.1 |
CHX/E-64d | 17.8 ± 1.1 | 18.2 ± 0.7 |
CHX/MG132/E-64d | 22.6 ± 0.5 | 22.6 ± 0.4 |
Data are means ± sd of three independent experiments in the presence of CHX, MG132, or E-64D as indicated. t1/2, half-life was quantified and analyzed by ImageJ after immunoblotting shown in Supplemental Figure 5 online.