Figure 5.

Influence of pI at the 14th Amino Acid of the 12th PPR.

(A) RNA gel blot analysis detecting petL operon RNA. The 14th amino acid of the 12th PPR motif was replaced by each amino acid indicated and then introduced into pgr3-1. WT, the wild type.

(B) Immunoblot detection of NdhL and PsaA.

(C) pI, molecular weight (MW), and hydrophobicity of each amino acid replaced. pI at pH 7.0 is indicated. Molecular weight is in daltons, hydrophobicity according to the Kyte-Doolittle scale (Kyte and Doolittle, 1982).