Figure 2.

Characterization of Two T-DNA Mutants in bZIP67.

(A) A diagram showing the position of T-DNA insertions in the bZIP67 gene. The bzip67-1 and bzip67-2 alleles are SALK_085497 and GK314D04, respectively. Exons are presented as black bars and introns as lines. The 5′ and 3′ untranslated regions are a white bar and a white arrow, respectively.

(B) PCR on bzip67-1 and bzip67-2 genomic DNA using primers spanning the T-DNA insertion sites in combination with T-DNA boarder primers (marked in [A]).

(C) RT-PCR on RNA isolated from whole developing siliques of bzip67-1, bzip67-2 and wild-type (WT) plants using bZIP67 primers that amplify the full-length cDNA (∼1 kb).

(D) RT-PCR on RNA isolated from whole developing siliques (both) and also separated silique and seed tissue of wild-type plants using bZIP67 primers that amplify the full-length cDNA. The PCR data are from single samples for each genotype but are representative of the results obtained from multiple independent experiments.