Figure 5.

Effect of bZIPs, L1L, and NF-YC2 on FAD3 Expression in the Wild Type, fus3-3, and abi3-7.

(A) Combined effect of bZIP67, L1L, and NF-YC2 transgenic expression in the wild type.

(B) Comparative effect of bZIP67, EEL, ABI5, and AREB3 transgenic expression in the wild type.

(C) Effect of bZIP67+L1L+NF-YC2 in abi3-7 and fus3-3. Mesophyll protoplasts were transfected with a FAD3 promoter-GUS reporter plasmid and effector plasmids for the indicated TFs. Transfected cells were cultured for 16 h and assayed for GUS and LUC activities. WT, the wild type.

Values are the mean ± se of measurements performed on three separate transfections and are normalized relative to LUC. Asterisk denotes a significant difference from the control (P < 0.05).