Figure 9.

MYC2 Binds to the Promoter of GS Biosynthesis Genes in Vivo.

ChIP-Seq was conducted on MYC2 using a MYC2-FLAG construct under native expression (Hou et al., 2010). Reads from high-throughput sequencing of immunoprecipitation from JA-treated plants (IP) and the mock experiment (mock) are shown. Screenshots from in-house genome browser (Anno-J, www.annoj.org) include four genes involved in the core biosynthetic pathways for indole-GSs ([A], CYP79B3; [B], SOT16) and aliphatic-GSs ([C], IPMDH1; [D], BCAT4).