Table 2. GLS Content of Micrografted Plants with Impaired Biosynthesis in the Rosette or Roots.

	GLS Biosynthesis		GLS Content of Entire Plant (% of the Wild Type)
Genotype (Rosette/Roots)	Rosette	Roots	Aliphatic GLS	Indole GLS	Total GLS
wt/wt	+	+	100	100	100
qko/qko	−	−	3.5 (±2.6)**	nd	1.8 (±1.3)**
wt/qko	+	−	28.8 (±5.1)**	20.7 (±3.8)**	23.8 (±3.5)**
qko/wt	−	+	50.7 (±4.4)**	56.6 (±23.3)*	53.6 (±12.0)**

Plants with impaired GLS synthesis in roots or the rosette were constructed by reciprocal micrografting of the myb28 myb29 cyp79b2 cyp79b3 quadruple mutant (qko) and Col-0 wild type (wt). GLS content of the entire 3-week-old grafted plants was analyzed by LC-MS and related to levels found in the entire wild type. Numbers are averages, and sd is shown in parentheses. Two-tailed t test, *P < 0.05 and **P < 0.01 versus the wild type (n = 5 to 7). nd, none detected.