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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Mol Aspects Med. 2013 May 15;37:3–14. doi: 10.1016/j.mam.2013.05.001

Figure 2.

Figure 2

Sodium butyrate increases the plasma membrane localization of Bsep-GFP. HEK293 cells were transfected with WT Bsep-GFP (A and B) or D482G-GFP (C and D) and confocal microscopy was used to monitor the plasma membrane expression of the constructs after treatment with sodium butyrate (NaB) for 24 hr. (A) DMSO control treated cells express WT Bsep-GFP primarily on the plasma membrane. (B) Similar localization was seen after NaB treatment. (C) Cells transfected with mutant D482G Bsep-GFP show little GFP fluorescence due to degradation of the protein (see details in (Lam et al., 2007). (D) Treatment with NaB resulted in stabilization of the protein and cells show increased plasma membrane expression of the D482G mutant of Bsep-GFP.