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Kinetoplast minicircle DNA detection by PCR. A 300- or 330-bp fragment (or both) amplified from T. cruzi minicircle DNA was present in all tested samples. M, DNA ladder marker (bp); lanes 1 through 7, pooled DNA pool samples (each pool contained 4 or 5 individual DNA samples that had been previously tested positive in T. cruzi real-time PCR); positive controls showing 330 bp (PC1) and 300- and 330-bp bands (PC2); nc, negative control (no-template control).
