FIGURE 2.

Sumoylation is differentially regulated during P. falciparum red blood cell stages. a, uninfected red blood cell lysate (RBC), 3D7 parasite lysate, and recombinant PfSUMO, HsSUMO-1, and HsSUMO-2, were analyzed using mAb 7E11 (α-PfSUMO). To demonstrate antibody specificity, lower portions of the blot were also probed with mAbs 21C7 (α-HsSUMO-1) and 8A2 (α-HsSUMO-2). b, immunoblot analysis of stage-specific P. falciparum parasite lysates using mAb 7E11 (α-PfSUMO). Detection of GFP-BCCP and Coomassie Blue staining of the immunoblot membrane are included as loading controls. c, indirect immunofluorescence microscopy of synchronized 3D7 parasites probed with anti-PfSUMO mAb 7E11 and anti-acyl carrier protein (ACP) antibodies. DNA was detected using DAPI. Bar indicates 2 μm.