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Ubc9 chimeras affect human and P. falciparum E1 enzyme interactions. a, schematic of wild type and chimeric Ubc9 proteins. b, assays were performed in the presence of human GFP-tagged SUMO-1, human E1-activating enzyme, and the indicted Ubc9 protein. c, assays were performed in the presence of human GFP-tagged SUMO-1, P. falciparum E1-activating enzyme, and the indicated Ubc9 protein. Reaction products were detected by immunoblot analysis with anti-GFP antibodies.
