FIGURE 6.

Exogenous inositol influences NAD⁺ levels in wild type cells grown in the presence of NA. NAD⁺ levels were measured as described under “Experimental Procedures” using the alcohol dehydrogenase assay. A, wild type, bna2Δ, and npt1Δ strains were grown continuously up to A_{600 nm} = 1 in I⁺NA⁺ and I⁻NA⁺ medium at 30 and 37 °C. B, wild type cells were grown in I⁺NA⁻ and I⁻NA⁻ at 30 and 37 °C and were harvested at A_{600 nm} = 1. Data represent the mean ± S.E. of at least three independent experiments, n = 3 or 4. The growth conditions indicated are: 30 °C (white bars) and 37 °C (black bars).