FIGURE 12.

Knockdown of cdk10 increased the mRNA level of raf1a, contributing to the impairment of NPC development. A, quantitative RT-PCR of the raf1a gene in control (Ctrl), cdk10 MO, MO + mRNA, and 5-mis MO in embryos at 24 HPF. Expression was normalized to ef1α. Knockdown of cdk10 significantly enhanced the transcription of raf1a. *, p < 0.05. Error bars represent S.D. B, embryos received the indicated MO with or without the MEK inhibitor U0126 and were collected at 80% epiboly, tail bud stage, or 24 HPF. Embryos were then subjected to whole-mount in situ hybridization using sox2 or ngn1 riboprobe. The severe diminution of sox2 and ngn1 expression observed in the cdk10 morphant was effectively eliminated by adding 10 μm U0126. Scale bars, 100 μm.