FIGURE 4.

Morphological phenotype of cdk10 morphants. A, phenotypic analyses of zebrafish embryos at 24 HPF. Embryos at one- to four-cell stages that received 1 ng of control MO (Ctrl), 1 ng of cdk10 MO, 1 ng of cdk10 MO plus 10 pg of cdk10 mRNA (MO + mRNA), and 1 ng of 5-mismatch cdk10 MO (5-mis) are depicted at 24 HPF. Compared with the normal morphology of the wild type (WT), cdk10 morphants exhibited head malformations with indistinct boundaries between the brain subdivisions as shown in the small insets. Images are shown in the lateral view with the anterior at the left. B, Western blot analysis of cdk10 knockdown efficiency. Embryos injected with the indicated MO were harvested at 24 HPF. Protein samples were prepared and probed using a human CDK10-specific antibody. β-Actin was used as the loading control. Data represent one of at least three independent experiments. C, percentages of normal, moderate, and severe phenotypes in the WT control (n = 159), cdk10 MO (n = 193), MO + mRNA (n = 279), and 5-mis MO (n = 178) embryos. Scale bar, 100 μm.