FIGURE 9.

Zebrafish cdk10 did not participate in cell cycle progression in vitro and in vivo. A, HEK 293 cell were transfected with pEGFP or pEGFP-cdk10. At 24 h post-transfection, cells were collected and subjected to flow cytometric analysis. The lower panel indicates the percentages of cells in the G₀/G₁, S, and G₂/M phases of the cell cycle. B, lateral views of cdkn1b, cdkn1c, ccnd1, and PCNA expression in embryos injected with control (Ctrl), cdk10 MO, MO + mRNA, and 5-mis MO at 24 HPF. Knockdown of cdk10 showed no apparent difference in cdkn1b, cdkn1c, ccnd1, and PCNA mRNA transcription, suggesting that cdk10 deficiency exhibited no influence on the progression of the cell cycle. Scale bars, 100 μm.