TABLE 3.
Effect of PK activity on PrPSc glycoform ratio
Values are expressed as relative percentage ± S.D. of diglycosylated (D), monoglycosylated (M), and unglycosylated (U) PrPSc based on densitometric analyses of immunoblots probed with mAb 3F4. Statistically significant comparisons (Student's t test or Mann-Whitney rank sum test) included diglycosylated PrPSc in VV1 (p < 0.01).
| CJD type | Lowest tested PK activitya |
PK activity at ED50b |
||||
|---|---|---|---|---|---|---|
| D | M | U | D | M | U | |
| VV1 | 21.0 ± 1.6 | 40.0 ± 4.0 | 39.0 ± 2.4 | 17.5 ± 1.0 | 40.7 ± 3.1 | 41.8 ± 3.4 |
| MM1 | 26.1 ± 1.5 | 49.0 ± 1.6 | 24.9 ± 2.3 | 25.9 ± 1.1 | 47.5 ± 2.4 | 26.6 ± 1.3 |
| MM 2T | 22.0 ± 0.4 | 44.5 ± 2.3 | 33.5 ± 1.9 | 20.3 ± 0.8 | 44.0 ± 0.5 | 35.7 ± 0.4 |
| MM 2C | 25.4 ± 2.0 | 42.0 ± 0.5 | 32.6 ± 1.9 | 24.6 ± 1.3 | 43.3 ± 2.4 | 32.1 ± 2.8 |
| MV 2K | 27.9 ± 1.9 | 40.6 ± 2.0 | 31.6 ± 0.5 | 27.5 ± 2.0 | 39.1 ± 0.8 | 33.4 ± 1.5 |
| VV2 | 39.1 ± 5.2 | 41.9 ± 1.5 | 19.0 ± 5.1 | 38.7 ± 3.9 | 41.2 ± 1.8 | 20.1 ± 3.5 |
| vCJD | 49.1 ± 0.7 | 34.5 ± 2.3 | 16.4 ± 1.6 | 47.0 ± 1.6 | 35.4 ± 1.9 | 17.7 ± 0.4 |
a Activity was 0.004 unit/ml for VV1 and MM 2T and 0.0315 unit/ml for all other subtypes.
b Activity was 0.0625 unit/ml for VV1, 0.125 unit/ml for MM1, 0.25 unit/ml for MM 2T and MM 2C, 2 units/ml for MV 2K, 5 units/ml for VV2, and 10 units/ml for vCJD.