FIGURE 2.

HDAC10 inhibits cervical cancer cell migration and invasion. A and B, HDAC10 overexpression and knockdown. A, HeLa cells were transfected with empty vectors or vectors encoding HDAC10 (left panel) and control siRNA or two siRNAs targeting HDAC10 (right panel). The cell lysates were analyzed by Western blotting with anti-HDAC10 antibodies. B, Caski cells were infected with lentivirus encoding HDAC10 (left panel) or short hairpin RNAs targeting HDAC10 (right panel). The expression of HDAC10 was analyzed by Western blotting with anti-HDAC10 antibodies. C and D, cell migration. HeLa cells (C) or Caski cells (D) of the control (con), HDAC10 overexpression, or HDAC10 knockdown group were collected and added to Transwell inserts. 16 h later, cells on the undersurface were fixed, stained, photographed, and counted. E and F, cell invasion. The upper surface of the inserts was precoated with Matrigel for 4 h. Control, HDAC10-overexpressing, and HDAC10 knockdown HeLa cells (E) and Caski cells (F) were added to the precoated Transwell chambers. Several hours later, cells were detected. Photos are shown on the left, and the dots are nuclei of the cells. Student's t test was performed, and the statistical analysis is shown on the right. Every experiment was repeated three times. *, p < 0.05; **, p < 0.01. Error bars represent S.D.