FIGURE 3.

Cooperation of clathrin with dynamin during membrane sorting and the modification of lipid rafts in B. abortus-infected phagocytes. HeLa cells were pretreated with 12.5 μM CPZ, 5 mM MβCD, or 80 μM dynasore (Dyn) or transiently transfected with control or clathrin siRNA prior to infection with B. abortus at an MOI of 10 for 10 min. Cell membranes were fractionated using sucrose density gradient ultracentrifugation. A and B, individual fractions were analyzed for clathrin (A) and dynamin (B) by immunoblotting. The lipid raft fractions are indicated by an asterisk in fraction 2. WB, Western blot.