FIGURE 6.

The role of clathrin in the recruitment of functional proteins associated with the endocytic pathway to BCVs. A, immunoblot analysis of clathrin, dynamin, caveolin, Rab5, F-actin, Arp2/3, and β-actin in BCVs isolated from HeLa cells pretreated with or without 12.5 μM CPZ at the indicated times p.i. The images shown are representative of three independent experiments. B, immunoblot ECL signals were quantified using National Institutes of Health ImageJ software, and the densitometry ratios of the β-actin signal to individual target protein signals are shown. Data represent the mean ± S.D. of triplicate trials from three independent experiments. Differences that were statistically significant compared with untreated samples are indicated. **, p < 0.01; ***, p < 0.001.