FIGURE 8.

Endostatin in RAC exosomes is responsible for suppression of retinal vessel leakage. A, endostatin level in the exosomes of RACs treated with or without FN-439 and RPE was examined by Western blotting using anti-endostatin Ab. B–E, PBS or exosomes derived from RACs treated with or without FN-439 were periocularly injected daily into mice with laser-induced CNV. After 7 days, retinal vascular leakage was determined (B and C), representative spot of CNV was photographed (D), and the average area of CNV in all four laser spots of six mice was measured (E) in choroidal flat mounts. **, p < 0.01 compared with control group treated with PBS in one-way ANOVA. Error bars indicate S.E.