FIGURE 2.
N-NusA AR1 interaction is not required for the antitermination process. Autoradiograms of the in vitro antitermination assays on the DNA templates carrying Rho-dependent (A; H-19B tR1 and λtR1) and Rho-independent (B; TR′-T1-T2) terminators in the presence of different NusA fragments. Termination zones and terminated products are indicated. RO means run-off product. %RT is defined under “Experimental Procedures.” C, different dilutions of H-19B and λ phages were spotted on the lawns of MG1655 having indicated derivatives of nusA supplied from a plasmid. Different NusA fragments and the source of N proteins are indicated against each figure. The NusA fragments are: WT, full-length; ΔAR2, 1–416 amino acids (amino acid); ΔAR1-2, 1–348 amino acids.