FIGURE 3.

Point mutations in NusA-NTD impair N-antitermination in vivo. A, the amino acid sequence surrounding the hydrophobic patch (italics) of the NusA-NTD. The numbering indicates the amino acid positions from the N terminus of NusA. The bar diagrams show the β-galactosidase activities from the reporter cassettes having Rho-dependent (B and D) and Rho-independent (C and E) terminators in the presence of the NusA-NTD mutants. Values obtained both in the absence and presence of H-19B N and λN are shown, and the comparison of −N and +N values gave the measure of in vivo antitermination. F, phage spotting efficiencies in the presence of the NusA-mutants. Experimental conditions were same as in Fig. 2C. Efficiency in the presence of WT NusA was set as 1. A.U., arbitrary units.