TABLE 1.

Oligonucleotide sequences

Restriction sites are in boldface type, whereas mutated sites are underlined.

MSMEG_2131_F	5′-GGAATTCCATATGAACGTGCTCTCCGCGGCGCT-3′
MSMEG_2131_R	5′-CCCAAGCTTTCCTTTCGATTCCTCCAACTGTC-3′
MSMEG_2131_K260A_F	5′-CGGCCTGATCGGCGCGTACTCGCGCCGG-3′
MSMEG_2131_K260A_R	5′-CCGGCGCGAGTACGCGCCGATCAGGCCG-3′
MSMEG_2131_K511A_F	5′-CACCTCTTCGGGCGCGCTGCGCCGCCTG-3′
MSMEG_2131_K511A_R	5′-CAGGCGGCGCAGCGCGCCCGAAGAGGTG-3′
MSMEG_2132_F	5′-GGAATTCCATATGCAGTCAACATCCCCTGA-3′
MSMEG_2132_R	5′-CCCAAGCTTTCACTGGTTGCTCTGCTCC-3′
Myokinase_F	5′-CCTCGGCCATATGCGTATCATTCTGCTTGG-3′
Myokinase_R	5′-GCCAAGCAGAATGATAAGCTTTTAGCCGAG-3′