FIGURE 2.

Mitochondrial coenzyme Q quantification. Mitochondrial samples were subjected to lipid extraction and HPLC-electrochemical detection to quantify quinone in the indicated culture media. A, coenzyme Q₆ determination in complemented strains was carried out in SDc-Ura and 2% (w/v) glucose to allow the vector selection by auxotrophy. Error bars represent S.D. a, p < 0.05 compared with WT. n = 3 in all the experiments. B, representative chromatograms. Growth in YPD, growth in YPG, and complementation of ptc7 with pCM189-PTC7 vector on SDc-Ura and 2% (w/v) glucose are shown. Q₆, coenzyme Q₆; Q₉, coenzyme Q₉.