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. 2013 Aug 12;288(39):28126–28137. doi: 10.1074/jbc.M113.474494

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Ptc7p dephosphorylates Coq7p in vivo. A and B, Coq7p phosphorylation in mutant ptc7 strain. V5-tagged versions of Coq7p were introduced into wild-type and mutant ptc7 yeast. Mitochondrial samples were isolated and analyzed. A, left, Coomassie Blue staining. Right, phosphoprotein determination by Pro-Q Diamond staining. Bottom, Coq7p-V5 immunodetection. M, molecular weight marker. B, mitochondrial samples were analyzed by Coq7p-V5 immunoprecipitation and two-dimensional isoelectric focusing analysis. Left, Pro-Q Diamond staining. Right, Coomassie staining. Bottom, Coq7p-V5 immunodetection.