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Aldose reductase catalyzes the reduction of carnosine-propanal conjugates. A, the ESI+/MS spectra of carnosine-propanal conjugates after incubation with AR. The carnosine-propanal conjugates were isolated by HPLC and incubated with recombinant AR and NADPH for 18–24 h. The ions generated in the presence of AR are indicated by an asterisk (*). Fragmentation patterns of the major ions with m/z values of 285 (B), 323 (C), and 341 (D) are shown.
