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. 2013 Aug 12;288(39):28207–28216. doi: 10.1074/jbc.M113.472464

FIGURE 4.

FIGURE 4.

Comparison of fluorescence spectra of 244B-opsin and M257Y/244B-opsin in DDM and nanodiscs. A, fluorescence emission spectra of 244B-opsin (black, λmax = 451 nm) and M257Y/244B-opsin (red, λmax = 456 nm) in DDM. B, fluorescence spectra of M257Y/244B-opsin in the absence (red, λmax = 456-nm) and presence (purple, λmax = 462-nm) of Gα-peptide in DDM. C, fluorescence spectra of 244B-opsin in the absence (black, λmax = 451 nm) and presence (purple, λmax = 451 nm) of Gα-peptide in DDM. D, fluorescence spectra of 244B-opsin (black, λmax = 454 nm) and M257Y/244B-opsin (red, λmax = 460 nm) in nanodiscs. E, fluorescence spectra of M257Y/244B-opsin in the absence (red, λmax = 460 nm) and presence (purple, λmax = 463 nm) of Gα-peptide in nanodiscs. F, fluorescence spectra of 244B-opsin in the absence (black, λmax = 454 nm) and presence (purple, λmax = 454 nm) of Gα-peptide in nanodiscs. The experiments were carried out at 10 °C.