TABLE 1.

Thermodynamic and dissociation constants for the p38α·DUSP16 MKBD interaction derived from ITC experiments at 25 °C

Experiments were performed in duplicate.

Interaction	Kd	ΔH	TΔS	ΔG
	μm	kcal × mol−1	kcal × mol−1	kcal × mol−1
p38α·DUSP16	0.89 ± 0.23	−1.25 ± 0.20	−7.0 ± 0.4	−8.26 ± 0.16
p38α·DUSP16H71A/S72A	1.94 ± 0.12	3.3 ± 0.4	−11.0 ± 0.4	−7.79 ± 0.03