FIGURE 4.

Activation of ChREBP and 14-3-3 interactions with βHB and AcAc. A, activation of FLAG-ChREBP·14-3-3β by varying concentrations of standard βHB and AcAc was determined using the pulldown assay as described in Fig. 1A. Representative images showing the 14-3-3 bound to ChREBP in varying βHB and AcAc are shown. B, βHB increased affinity of 14-3-3β binding to ChREBP. Varying concentrations of 14-3-3β to FLAG-ChREBP were added to the reaction mixture in the presence of 0, 0.1, or 1 mm βHB, and 14-3-3β bound to FLAG-ChREBP beads was subjected to the pulldown assay.