TABLE 1.

Thermodynamic parameters for the interactions of human 14-3-3 proteins with human ChREBP peptides α₂-Ser-140p and α₃-Ser-196p in the presence of βHB as determined by isothermal titration calorimetry

Data represent at least two ITC measurements.

Peptides and β-hydroxybutyrate	Kd
	m
α2-Ser-140p	1.1 × 10−6
α2-Ser-140p + 10 μm βΗΒ	1.5 × 10−6
α2-Ser-140p + 100 μm βΗΒ	0.71 × 10−6
α2-Ser-140p + 1000 μm βΗΒ	0.60 × 10−6
α3-Ser-196p	0.78 × 10−6
α3-Ser-196p + 10 μm βHB	0.83 × 10−6
α3-Ser-196p + 100 μm βΗΒ	0.63 × 10−6
α3-Ser-196p + 1000 μm βΗΒ	0.59 × 10−6