FIGURE 4.

PDZ1-CT interactions. A, stereoview of the PDZ1-CT interface in the GRASP65 structure. A ribbon representation of the PDZ1 domain is shown in blue, and the stick representation of the CT is colored in cyan. Relevant amino acids are indicated. The residue from the CT is labeled with an asterisk. B, same as described for A, but with the GRASP55 structure. A ribbon representation of the PDZ1 domain is shown in dark green, and the stick representation of the CT is colored in light green. C, HeLa cells were transfected with wild-type (wt) or mutant GRASP65-Myc and treated in the absence or presence of 400 μm EGS. The lysates were analyzed by SDS-PAGE and immunoblotting with anti-Myc antibodies. Asterisks indicate the number of GRASP65 molecules in the cross-linked product. The black arrowhead indicates degraded GRASP65, and the white arrowhead indicates cross-linked product containing degraded protein. The levels of β-actin were monitored as a loading control. D, same as described for C, but with wild-type or mutant GRASP55-Myc.