FIGURE 6.

Golgi membrane stacking mediated by the GRASP proteins. Wild-type (wt) or mutant GRASP proteins were subjected to the BFA sensitivity assay. BFA concentrations of 5 and 0.5 μg/ml were used for GRASP65 and GRASP55, respectively. A–D, mutations related to the PDZ2-PDZ2 interface. E–G, mutations related to the peptide-binding pocket of the PDZ1 domain. H and I, mutations related to the CT. For each sample, at least 300 cells were categorized as described in the legend to Fig. 5A. All graphs are representative of three repetitions. See also supplemental Fig. S4.