Table 1.

Primers and probes

Gene	Forward primer (5’-3’)*	Reverse primer (5’-3’)*	5’ fluorophore†	Probe (5’-3’)*	3’ quencher‡	Amplicon length (bp)
HIF1α	agcagtctatttatattttctaca	agagcattaatgtaaattaagtag	FAM	tagaagcctggctacaatactgca	BHQ1	125
CD68	caatggttcccagccctgtg	tccctggaccttggttttgttg	FAM	ccacctccaagcccagattcagattcgag	BHQ1	134
TBP	ggttgtaaacttgacctaaag	gttcgtggctctcttatc	FAM	tgattaccgcagcaaaccgc	BHQ1	134
RPLP	gacggattacaccttccc	gactcttccttggcttca	Cy5	ccttcttggctgatccatctgc	BHQ2	139

^*Nucleic acid sequence of primers and probes.

^†Fluorophores used: Cy5; Cyanine fluorophore, FAM; Fluorescein amidite.

^‡Quenchers are non-fluorescent chromophores quenching non-hydrolysed probes by fluorescence resonance energy transfer (FRET): BHQ1; black hole quencher 1 deoxythymidine, BHQ2; black hole quencher 2 deoxythymidine.