Fig. 5.

Src is required for mechanical activation of TACE phosphorylation at Tyr702. C2C12 myoblasts were transfected with Src-specific siRNA or scrambled siRNA as control, and stretched for 30 minutes to assess Src activity and TACE phosphorylation at Tyr702 (A), 2 hours to assess p38 activation (B) or 3 hours to assess myogenin and p21 expression (C) using western blotting. Optical density data were analyzed by ANOVA. *P<0.05 compared with the non-stretched myoblasts (0 minute) that were transfected with control siRNA, **P<0.05 compared with the stretched myoblasts that were transfected with control siRNA.