Fig. 6.

Mechanically activated Src and TACE interact and colocalize in clusters. (A) Mechanical stretch induces interaction between Src and TACE. C2C12 myoblasts were stretched for the indicated time period. Cell lysate was subjected to immunoprecipitation with an antibody against TACE and analyzed by western blotting for Src co-precipitation with TACE. (B) Mechanical stretch induces colocalization of Src and TACE into clusters. C2C12 myoblasts were stretched for 30 minutes and then subjected to immunofluorescence staining with antibodies against pY416-Src (red) and pY702-TACE (green). Scale bar: 20 µm. The Pearson coefficient for the colocalization in control and stretched cells was analyzed by Student's t-test, *P<0.01 compared with the non-stretched control.